Experiment 1: % input vehicle 2.34, drug 2.02 (ratio 0.86); IgG % input 0.031 and 0.064; fold over IgG vehicle 76, drug 32
Experiment 2: % input vehicle 0.92, drug 0.98 (ratio 1.06); IgG % input 0.067 and 0.025; fold over IgG vehicle 14, drug 39
Experiment 3: % input vehicle 2.82, drug 2.12 (ratio 0.75); IgG % input 0.045 and 0.039; fold over IgG vehicle 63, drug 54
Experiment 4: % input vehicle 2.36, drug 3.14 (ratio 1.33); IgG % input 0.049 and 0.079; fold over IgG vehicle 49, drug 40
A. qPCR wells as n (12 vs 12), Welch t-test on % input: drug / vehicle 0.98, p = 0.91
B. Experiments as n but unpaired (4 vs 4), Welch t-test on log2 % input: drug / vehicle 0.98, p = 0.95
C. Log2 ratio of % input per experiment (paired, n = 4): drug / vehicle 0.98 (95% CI 0.66 to 1.45), p = 0.87
D. Log2 ratio of fold enrichment over IgG per experiment (paired, n = 4): drug / vehicle 0.95 (95% CI 0.27 to 3.34), p = 0.91