Transfection 1: firefly / Renilla control 4.23, treated 3.72 (fold 0.88); Renilla treated / control 0.77
Transfection 2: firefly / Renilla control 3.48, treated 3.61 (fold 1.04); Renilla treated / control 1.41
Transfection 3: firefly / Renilla control 4.50, treated 4.42 (fold 0.98); Renilla treated / control 0.66
Transfection 4: firefly / Renilla control 3.90, treated 3.17 (fold 0.81); Renilla treated / control 0.76
A. Firefly alone, log2 ratio per transfection (paired, n = 4): treated / control 0.80 (95% CI 0.42 to 1.52), p = 0.35
B. Firefly / Renilla, wells as n (12 vs 12), Welch t-test: treated / control 0.93, p = 0.21
C. Firefly / Renilla, log2 ratio per transfection (paired, n = 4): treated / control 0.92 (95% CI 0.78 to 1.10), p = 0.24
D. Check: Renilla alone, log2 ratio per transfection (paired, n = 4): treated / control 0.86 (95% CI 0.50 to 1.47), p = 0.44