Weighted 4PL: bottom 0.0512, top 2.7986, EC50 147.27 pg/mL, slope 1.2151
Back-calculated standards (mean of duplicate wells), % of nominal; CV of the duplicate ODs:
   1000.00 pg/mL:  122.1%  ok (limit +/-25%); CV 1.3%
    500.00 pg/mL:   89.1%  ok (limit +/-20%); CV 4.7%
    250.00 pg/mL:  104.3%  ok (limit +/-20%); CV 1.3%
    125.00 pg/mL:   98.5%  ok (limit +/-20%); CV 3.2%
     62.50 pg/mL:  100.7%  ok (limit +/-20%); CV 0.2%
     31.25 pg/mL:  101.1%  ok (limit +/-20%); CV 1.5%
     15.62 pg/mL:   98.4%  ok (limit +/-20%); CV 1.7%
      7.81 pg/mL:  100.9%  ok (limit +/-25%); CV 3.1%
Straight line through all standards, back-calculated % of nominal:
  1000: 83%, 500: 135%, 250: 213%, 125: 227%, 62.5: 124%, 31.25: -217%, 15.625: -962%, 7.8125: -2395%
Samples (mean OD of duplicate wells), reportable range 7.8125 to 1000 pg/mL:
  S1: OD 0.0951 -> below the LLOQ (report as < 7.8125 pg/mL)
  S2: OD 0.2693 -> 19.6 pg/mL
  S3: OD 0.9247 -> 78.6 pg/mL
  S4: OD 2.0565 -> 333.7 pg/mL
  S5: OD 2.2027 -> 423.6 pg/mL, but on the upper plateau (dilute and re-assay)
  S6: OD 2.6053 -> above the ULOQ (dilute and re-assay)