Experiment 1, WT, nonlinear regression: Km 10.77 uM (95% CI 9.62 to 11.93); Vmax 103.1 (95% CI 98.6 to 107.5)
Experiment 1, WT, Lineweaver-Burk: Km 13.76 uM; Vmax 118.0
Km (uM) in each experiment, nonlinear regression [Lineweaver-Burk]:
  Experiment 1: WT 10.77 [13.76]; mutant 16.14 [14.49]
  Experiment 2: WT 8.99 [12.59]; mutant 15.05 [24.26]
  Experiment 3: WT 12.12 [18.27]; mutant 14.88 [91.48]
  Experiment 4: WT 9.78 [9.59]; mutant 15.90 [15.30]
  Experiment 5: WT 10.67 [12.43]; mutant 19.08 [16.64]
Km ratio, mutant / WT, one ratio per experiment (paired t-test on log Km, n = 5): 1.55 (95% CI 1.29 to 1.86), p = 0.003
Global fit of all 160 wells (a Vmax for each preparation, Km shared across experiments): Km WT 10.3 (95% CI 9.6 to 11.0), mutant 15.9 (95% CI 14.7 to 17.2); extra-sum-of-squares F(1, 148) = 73.88, p = 1.1e-14