Nonlinear regression: median Km 10.00 uM; middle 95% of estimates 8.49 to 11.71; within 20% of the true 10 uM in 98.5%; negative or above 100 uM in 0.0%
Lineweaver-Burk: median Km 10.34 uM; middle 95% of estimates 6.61 to 22.57; within 20% of the true 10 uM in 54.1%; negative or above 100 uM in 0.1%
Same Km in both enzymes (10 uM): p<0.05 with one Km ratio per experiment (paired t-test, n = 5) 4.8%; with a global fit sharing Km across experiments (extra-sum-of-squares F-test) 20.0%
Mutant Km 1.5 times higher (15 uM): p<0.05 with one Km ratio per experiment (paired t-test, n = 5) 93.6%; with a global fit sharing Km across experiments (extra-sum-of-squares F-test) 100.0%