Cells: 381, CTCF median 586,159, smallest 80,522; background is 25% of the raw integrated density A. Cells as n (185 vs 196), Welch t-test on log CTCF: knockdown / control 0.96, p = 0.47 B. Images as n (20 vs 20), Welch t-test on mean log CTCF: knockdown / control 0.97, p = 0.79 C. Experiments as n, CTCF, log ratio per experiment (n = 4): knockdown / control 0.97 (95% CI 0.77 to 1.23), p = 0.74 D. Experiments as n, raw integrated density (no background subtraction), log ratio per experiment (n = 4): knockdown / control 0.97 (95% CI 0.82 to 1.14), p = 0.58