Experiment 1: control Bmax 443 fmol/mg, Kd 1.08 nM [Scatchard 1.09]; treated Bmax 329, Kd 1.07 [Scatchard 1.10]
Experiment 2: control Bmax 525 fmol/mg, Kd 1.15 nM [Scatchard 1.11]; treated Bmax 429, Kd 1.09 [Scatchard 1.01]
Experiment 3: control Bmax 614 fmol/mg, Kd 0.93 nM [Scatchard 0.88]; treated Bmax 352, Kd 0.92 [Scatchard 0.90]
Experiment 4: control Bmax 573 fmol/mg, Kd 1.36 nM [Scatchard 1.29]; treated Bmax 315, Kd 1.09 [Scatchard 1.12]
Experiment 5: control Bmax 540 fmol/mg, Kd 0.89 nM [Scatchard 0.95]; treated Bmax 330, Kd 1.07 [Scatchard 1.20]
Bmax, treated / control, one ratio per experiment (paired, n = 5): 0.65 (95% CI 0.53 to 0.81), p = 0.005
Kd, treated / control, one ratio per experiment (paired, n = 5): 0.98 (95% CI 0.82 to 1.17), p = 0.76
Control pKd: mean 8.97 (SD 0.07); geometric mean Kd 1.07 nM
Global fit of all 240 wells (Kd shared across experiments within each condition): Kd treated / control 1.00; extra-sum-of-squares F(1, 228) = 0.00, p = 0.95