Cells as replicates (1000 vs 1000 cells), Wilcoxon, BH q < 0.05: 63 genes (5 of the 5 truly changed, 58 of the 95 unchanged)
Pseudobulk (4 vs 4 mice), Welch's t-test on log2 CPM, BH q < 0.05: 0 genes (0 of the 5 truly changed, 0 of the 95 unchanged)
Pseudobulk log2 fold change, genes 001-005: 0.51, 1.09, 0.73, 0.84, 0.82; largest absolute value among the 95 unchanged genes 0.77
Pseudobulk, five smallest p-values: 003 (p = 0.025), 002 (p = 0.027), 012 (p = 0.037), 001 (p = 0.039), 004 (p = 0.040)