Experiment 1: control 66.3 cells per field, knockdown 57.8, knockdown 87% of control
Experiment 2: control 63.7 cells per field, knockdown 53.2, knockdown 84% of control
Experiment 3: control 52.2 cells per field, knockdown 33.6, knockdown 64% of control
Experiment 4: control 40.7 cells per field, knockdown 55.9, knockdown 137% of control
A. Fields as n (40 vs 40), Welch t-test: knockdown / control 0.90, p = 0.26
B. Inserts as n (8 vs 8), raw counts, Welch t-test: knockdown / control 0.90, p = 0.45
C. Percent of control, control fixed at 100% (n = 4 per group), Student's t-test: knockdown 93%, p = 0.67
D. Percent of control, one-sample t-test against 100% (n = 4): knockdown 93%, p = 0.69
E. Log ratio per experiment (paired, n = 4): knockdown / control 0.90 (95% CI 0.54 to 1.48), p = 0.53